RBS

Part:BBa_J100335:Design

Designed by: Jack Dougherty   Group: Campbell M Lab   (2017-10-19)


Attempt at the Creation of a Strong RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We had to consider which bases and nucleotide combinations were essential to conserve and which were best to use for variation through mutational analysis.


Source

Here is the website for the RBS Calculator: https://www.denovodna.com/software/ Here is the link to the original part we used: https://parts.igem.org/Part:BBa_J119384


References